Cas9 nuclease

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CRISPR/Cas systems are originally discovered in bacteria and archaea as an adaptive immune system to defense against viruses and plasmids in the enviroment. In this system, Cas9 (CRISPR associated protein 9) protein is guided by a non-conding RNA (gRNA) to cut the double-strand DNA at the desired genomic location. Then, endogenous DNA repairing processes are activated to heal the targeted DSB.

Recombinant Streptococcus pyogenes Cas9 protein is overexpressed in E.coli, and purified with N- and C-terminal NLS signal as a ready-to-injection/transfection reagent for genome engineering experiments.










Expressed in


In vitro assay



Store all components at -20°C

Storage buffer

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycero

10 x reaction buffer

200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5


For laboratory research only. Not for clinical applications.

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