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CRISPR/Cas systems are originally discovered in bacteria and archaea as an adaptive immune system to defense against viruses and plasmids in the enviroment. In this system, Cas9 (CRISPR associated protein 9) protein is guided by a non-conding RNA (gRNA) to cut the double-strand DNA at the desired genomic location. Then, endogenous DNA repairing processes are activated to heal the targeted DSB. |
Cas9 protein is overexpressed in E.coli, and purified with N- and C-terminal NLS signal. Cy3 labeled Cas9 is a ready-to-injection/transfection reagent for genome engineering experiments, and enable for sorting and visualization of the transfected cells. The fluorescent signals can be detected by confocal microscope.
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Alternative name |
Cy3-spCas9 |
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Expressed in |
E.coli |
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In vitro assay |
 |
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Storage |
Store all components at -20°C |
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Storage buffer |
10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycero |
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10 x reaction buffer |
200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5 |
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Note |
For laboratory research only. Not for clinical applications. |
