Cy3-Cas9 nuclease

In stock
Bulk Inquiry

CRISPR/Cas systems are originally discovered in bacteria and archaea as an adaptive immune system to defense against viruses and plasmids in the enviroment. In this system, Cas9 (CRISPR associated protein 9) protein is guided by a non-conding RNA (gRNA) to cut the double-strand DNA at the desired genomic location. Then, endogenous DNA repairing processes are activated to heal the targeted DSB.

Cas9 protein is overexpressed in E.coli, and purified with N- and C-terminal NLS signal. Cy3 labeled Cas9 is a ready-to-injection/transfection reagent for genome engineering experiments, and enable for sorting and visualization of the transfected cells. The fluorescent signals can be detected by confocal microscope.

For technical questions, please email us at

For bulk order, please contact

Alternative name


Expressed in


In vitro assay



Store all components at -20°C

Storage buffer

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycero

10 x reaction buffer

200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5


For laboratory research only. Not for clinical applications.


Write Your Own Review
You're reviewing:Cy3-Cas9 nuclease
Your Rating