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Catalog # |
Size |
Product Type |
|
C1003 |
2 vials (for 4 96-well plates) |
Cell line |
|
Alternative name |
3T3-msCD40L, 3T3-CD40L treated by irradiation |
|
Description |
3T3-CD40L is a mouse fibroblast cell line that secretes mouse CD40L. 3T3-CD40L cells can be co-cultured with B cells to aid B cell expansion. Irradiated 3T3-msCD40L cells are treated by irradiation, and ready for feeding B cell culture. |
|
Cell Type |
Mouse 3T3 cell line expression CD40L |
|
Accession ID |
CVCL_1H10 |
|
Organism |
Mouse |
|
Biosafety Level |
2 |
|
Subculturing |
Adherent Cells, like fibroblast cells |
|
Maintenance Tips |
Irradiated 3T3-CD40L cells are used for feeding B cell for once, and cannot be maintained for long term. |
|
Propagation Medium |
DMEM with 10% FBS, 1% L-glutamine, and 0.1% gentamicin. |
|
Reference |
J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013) |
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Note |
For laboratory research only. Not for clinical applications. For technical questions, please email us at support@cellron.com or visit our website at www.cellron.com
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