Custom Protein Services
Custom Protein Services
Multiple expression system: Bacterial, yeast, insect cells, mammalian cells
Featured cell free expression system
Various modification labels for the purified protein, including fluorescence dyes, biotinylation
Reliable, professional, PhD level expertise
Cost effective and time saving one-step service
Boston Cellron provides professional custom protein service. Our one-step service covers all you need from gene synthesis, expression screen, multiple purification methods, and post modification, including fluorescence labels and other conjugated tags. If you are looking for proteins in your research, please contact with our technical support suppot@cellron.com. We will help you to choose the appropriate expression system and purification method, and coordinate with you by updating each milestone phase reports.
Outline of Recombinant Protein Expression Service in Bacteria
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Phase Service |
Timeline |
Description |
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Phase 1: Gene cloning |
1-2 week |
Use your expression vectors without any modification |
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Gene synthesis and codon optimization |
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Subclone your gene into an expression vector |
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Phase 2: Large-scale gene expression |
1 week |
Transform expression plasmid into bacteria |
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Express protein in 1 liters of culture |
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Collect, lyse and extract the induced cells |
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Centrifuge to obtain the supernatant fractions for purification |
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Phase 3: Protein affinity purification |
1-2 week |
Protein affinity chromatography purification |
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Determine the protein purity by SDS-PAGE with Coomassie blue staining |
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Protein desalting |
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Determine the protein concentration |
Outline of Recombinant Protein Expression Service in Mammalian Cells
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Phase Service |
Timeline |
Description |
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Phase 1: Gene cloning |
1.5 weeks |
Use your expression vectors without any modification |
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Gene synthesis and codon optimization |
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Provide gene template or subclone your genes into either an expression vector provided by you or an overexpression vector from Boston Cellron |
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Phase 2: Gene Expression |
1-3 weeks |
Small scale of gene expression: transfect 2 ml of HEK293 suspension cell cultures using the sterile recombinant plasmid DNA |
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SDS-PAGE and Western blotting analysis to confirm the gene expression, expression levles, protein secretion, protein solubility, etc |
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Upon request, perform large scale of gene transfection (100-500 ml) |
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Centrifuge to obtain the culture supernatant and cell pastes for purification |
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Phase 3: Protein affinity purification |
2 weeks |
Cell lysis, protein extraction, centrifugation, etc. |
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Protein affinity chromatography purification from lysate and/or cel culture supernatant, desatling, etc. |
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Determine the protein purity by SDS-PAGE with Coomassie blue staining |
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Determine the protein concentration. Concentrate the samples if protein concentration is too low |
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QC process, deliver protein product and project report, etc. |
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