E.coli Protein Service

E.coli Protein Services

The E. coli expression system is the most widely used expression system for recombinant proteins with several advantages, including low cost, high expression level, ease of scaling, and a short turnaround time.

Outline of Recombinant Protein Expression Service in Bacteria

Phase Service	Timeline	Description
Phase 1: Gene cloning	1-2 week	Use your expression vectors without any modification
		Gene synthesis and codon optimization
		Subclone your gene into an expression vector
Phase 2: Large-scale gene expression	1 week	Transform expression plasmid into bacteria
		Express protein in 1 liters of culture
		Collect, lyse and extract the induced cells
		Centrifuge to obtain the supernatant fractions for purification
Phase 3: Protein affinity purification	1-2 week	Protein affinity chromatography purification
		Determine the protein purity by SDS-PAGE with Coomassie blue staining
		Protein desalting
		Determine the protein concentration