Mammalian Cell Expression Service

Protein expression with the mammalian cell expression system has the most comprehensive post-translational modifications with guaranteed activity. We provide 2 expression options: transcient transfection and stable cell line expression.

Outline of Recombinant Protein Expression Service in Mammalian Cells

Phase Service	Timeline	Description
Phase 1: Gene cloning	1.5 weeks	Use your expression vectors without any modification
		Gene synthesis and codon optimization
		Provide gene template or subclone your genes into either an expression vector provided by you or an overexpression vector from Boston Cellron
Phase 2: Gene Expression	1-3 weeks	Small scale of gene expression: transfect 2 ml of HEK293 suspension cell cultures using the sterile recombinant plasmid DNA
		SDS-PAGE and Western blotting analysis to confirm the gene expression, expression levles, protein secretion, protein solubility, etc
		Upon request, perform large scale of gene transfection (100-500 ml)
		Centrifuge to obtain the culture supernatant and cell pastes for purification
Phase 3: Protein affinity purification	2 weeks	Cell lysis, protein extraction, centrifugation, etc.
		Protein affinity chromatography purification from lysate and/or cel culture supernatant, desatling, etc.
		Determine the protein purity by SDS-PAGE with Coomassie blue staining
		Determine the protein concentration. Concentrate the samples if protein concentration is too low
		QC process, deliver protein product and project report, etc.