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Catalog # |
Quantity |
Price |
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P1016 |
2,000IU/10,000IU/50,000IU |
$50/200/600 |
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Alternative name |
rTEV, TEV, P1 Protease |
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Description |
TEV (Tobacco Etch Virus Protease) is a highly site-specific protease purified from E.coli. The specific protein sequence recognized by TEV is ENLYFQ (G/S), and the cleavage site is between Q and G/S residues. This protease is used to cleave affinity tags, such as MBP tag or poly-His tag from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more TEV.
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Expressed in |
E.coli |
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Molecular Weight |
28.4 kDa |
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Biological Activity |
5 IU/μl. |
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Storage |
1 year at -80°C from the date of shipment. Please avoid freeze-thaw cycles. |
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Formulation |
Supplied as a >=2 mg/ml in 25 mM Tris-HCl, pH 8.0, 150 mM NaCl with 50% glycerol |
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Purity |
≥95%, determined by SDS-PAGE |
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Note |
For laboratory research only. Not for clinical applications. |
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For technical questions, please email us at support@cellron.com. For bulk order, please contact sales@cellron.com. |
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